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KMID : 0380420010250010041
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Journal of Prventive Veterinary Medicine
2001 Volume.25 No. 1 p.41 ~ p.46
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Simultaneous Determination of Water-Soluble Vitamins in Infant Dried Milk Formulations by High Performance Liquid Chromatography with UV Detector
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박성원/Park, Sung Won
이득신/손성완/김재훈/조남인/김옥경/Lee, Deuk Shin/Son, Seong Wan/Kim, Jae Hoon/Jo, Nam In/Kim, Ok Kyung
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Abstract
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An ion pair liquid chromatographic method is described for the simultaneous determination of water-soluble vitamins C, B1, B2, B6 and nicotinamide in dried milk infant formulations. Commercial infant formulations and water-soluble vitamin spiked samples of the formulations were extracted with distilled water consisting of 5mM of hexane sulfonic acid sodium salt, 2% meta[hosphoric acid and 1％ acetic acid and filtrated. And the subsequent analysis of the filtrate was performed by liquid chromatography. The filtrate contained water-soluble vitamins were free from interfering compounds when analyzed by liquid chromatography with UV detection (variable wavelength, 270 & 290nm). Gradient elution was required to elute vitamin C, B1, B2, B6 and nicotinamide from a C18 reversed phase column using the mobile phase consisting of 0 to 60％ methanol contained 1％ acetic acid and the counter ion, 5mM hexane sulfonic acid. Correlation coefficients of standards curves for individual vitamin from standard solution were linear (from 0.9985 to 0.9999) with average percentage recoveries from 85.0 to 116.9 for the concentration range (1.0∼400μg/g) examined.
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